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Registros recuperados: 28
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Inter-specific and ontogenic differences in delta C-13 and delta N-15 values and Hg and Cd concentrations in cephalopods ArchiMer
Chouvelon, Tiphaine; Spitz, Jerome; Cherel, Y.; Caurant, Florence; Sirmel, R.; Mendez-fernandez, P.; Bustamante, Paco.
Chemical tracers (e. g. stable isotopes or trace metals) are increasingly used to study trophic ecology and feeding habits of marine organisms and various factors can affect their values. The aim of the present study was to provide information on ontogenic effects on stable carbon and nitrogen values (delta C-13 and delta N-15) and on cadmium (Cd) and mercury (Hg) concentrations in 5 cephalopod species from the Bay of Biscay (North-East Atlantic Ocean). To this end, individuals belonging to 3 species showing the widest range of sizes were analysed for muscle delta C-13 and delta N-15 values and muscle Hg, and digestive gland Cd concentrations. Results showed that stable isotope ratios allowed discrimination of specific feeding strategies during...
Tipo: Text Palavras-chave: Stable isotopes; Metals; Trace elements; Ontogenesis; Prey; Ecological tracer; Northeastern Atlantic.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00166/27761/25938.pdf
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Prey preferences of adult sea bass Dicentrarchus labrax in the northeastern Atlantic: implications for bycatch of common dolphin Delphinus delphis ArchiMer
Spitz, Jerome; Chouvelon, Tiphaine; Cardinaud, M.; Kostecki,; Lorance, Pascal.
In the northeastern Atlantic, adult sea bass (Dicentrarchus labrax) is one of largest fish living on the shelf, and this species has important commercial value. However, pelagic trawl fisheries that target sea bass have negative operational interactions with common dolphins (Delphinus delphis). Our goal was to determine the diet of adult sea bass in the Bay of Biscay from stomachcontent and stable-isotope analyses, and explore the dietary overlap between sea bass and common dolphins. We found that sea bass primarily target small pelagic fish, most notably mackerel (Scomber scombrus), scads (Trachurus spp.), anchovy (Engraulis encrasicolus), and sardine (Sardina pilchardus). These four species also dominated the diets of common dolphins. This overlap in...
Tipo: Text Palavras-chave: Chesson's index; Marine top predator; Prey selection; SIAR; Stable isotope; Stomach content.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00125/23625/21485.pdf
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Evolution temporelle du comportement estuarien du mercure: cas de l'estuaire de la Loire ArchiMer
Knoery, Joel; Brach Papa, Christophe; Thomas, Bastien; Auger, Dominique; Chouvelon, Tiphaine; Crochet, Sylvette; Rozuel, Emmanuelle; Sanjuan, Jane; Thibault, Aubin; Chiffoleau, Jean-francois.
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00212/32356/30812.pdf
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Trophic ecology of European sardine Sardina pilchardus and European anchovy Engraulis encrasicolus in the Bay of Biscay (north-east Atlantic) inferred from delta C-13 and delta N-15 values of fish and identified mesozooplanktonic organisms ArchiMer
Chouvelon, Tiphaine; Chappuis, A.; Bustamante, Paco; Lefebvre, Sebastien; Mornet, Francoise; Guillou, G.; Violamer, L.; Dupuy, Christine.
European sardine (Sardina pilchardus) and European anchovy (Engraulis encrasicolus) are two species of economical and ecological significance in the Bay of Biscay (north-east Atlantic). However, the trophic ecology of both species is still poorly known in the area, and more generally, few studies have considered the potential trophic overlap between sardines and anchovies worldwide. This study aims to highlight the trophic links between the mesozooplankton and adults of these two pelagic fish in the Bay of Biscay, through carbon and nitrogen stable isotope analysis (SIA). Mesozooplankton and individuals of sardines and anchovies were collected during one season (spring 2010), over spatially contrasted stations within the study area. First, the potential...
Tipo: Text Palavras-chave: Plankton preservation; Plankton delipidation; Pelagic fish; Trophic interactions; Spatial variability; Isotopic mixing model.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00176/28741/27280.pdf
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Monitoring of Pb Contamination in Loire Estuary: Trends, Distribution and Isotopic composition ArchiMer
Brach-papa, Christophe; Chiffoleau, Jean-francois; Knoery, Joel; Chouvelon, Tiphaine; Auger, Dominique; Bretaudeau-sanjuan, Jane; Crochet, Sylvette; Rozuel, Emmanuelle; Thomas, Bastien; Vasileva, Emilia; Oriana, Anna Maria.
The Loire River is one of the largest river systems in Western Europe and a major source of continental inputs to the marine environment in the Bay of Biscay. Its catchment area drains agricultural and industrial zones and its estuary is increasingly urbanized. Even if the Loire River is not considered as a highly polluted system, studies have shown estuarine Pb contamination due to industrial inputs and combustion of leaded gasoline until the mid 1990’s. A retrospective study, based on the analysis (Pb levels and isotopic composition) of mussel samples collected by the French mussel watch program (RNO/ROCCH) has highlighted this contamination and its trend between 1985-2005 (Couture et al., 2010). This poster furthers the work initiated by Couture et al....
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00188/29958/28404.pdf
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Small pelagic fish feeding patterns in relation to food resource variability: an isotopic investigation for Sardina pilchardus and Engraulis encrasicolus from the Bay of Biscay (north-east Atlantic) ArchiMer
Chouvelon, Tiphaine; Violamer, L.; Dessier, Aurelie; Bustamante, Paco; Mornet, Francoise; Pignon-mussaud, Cecilia; Dupuy, Christine.
Small pelagic fish represent an essential link between lower and upper trophic levels in marine pelagic ecosystems and often support important fisheries. In the Bay of Biscay in the north-east Atlantic, no obvious controlling factors have yet been described that explain observed fluctuations in European sardine Sardina pilchardus and European anchovy Engraulis encrasicolus stocks, in contrast to other systems. The aim of this study was therefore to investigate to which extent these fluctuations could be trophodynamically mediated. The trophic ecology of both fish species was characterised over three contrasting periods (spring 2010 and 2011 and autumn 2011) in the area, in relation to potential variation in the abundance and composition of the...
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Ano: 2015 URL: https://archimer.ifremer.fr/doc/00247/35859/34381.pdf
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Projet GERMON. Structure génétique et migration du thon Germon ArchiMer
Nikolic, Natacha; Puech, Alexis; Chouvelon, Tiphaine; Munschy, Catherine; Bodin, Nathalie; Brach-papa, Christophe; Potier, Michel; West, Wendy; Knoery, Joel; Zudaire, Iker; Dhurmeea, Zahirah; Degroote, Maxime; Cedras, Maria; Evano, Hugues; Bourjea, Jerome.
The GERMON project had several aims i) to understand the populations’ structure between albacore tuna southwest of the Indian Ocean and southeast Atlantic, ii) to determine the origin of juveniles observed in South Africa iii) to improve the understanding of the biology field (Size/Size and Size/Weight; reproduction and feeding area; trophic linkages) and iv) to initiate work on chemical analyzes and adaptation of the albacore species to its environment. These aims have been held and the results allow a better understanding of the management albacore. A general summary of results is presented in Part 9.
Tipo: Text Palavras-chave: Germon; Thunnus alalunga; Génétique; Stock; Structure; Connectivité; Chimie; Reproduction; Alimentation; Habitat; Albacore; Thunnus alalunga; Genetic; Stock; Structure; Connectivity; Chemical; Reproduction; Feeding behavior; Habitat.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00293/40461/39152.pdf
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Isotope and fatty acid trends along continental shelf depth gradients: Inshore versus offshore hydrological influences on benthic trophic functioning ArchiMer
Chouvelon, Tiphaine; Schaal, G.; Grall, J.; Pernet, Fabrice; Perdriau, M.; A-pernet, E. J.; Le Bris, H..
Anthropogenic activities and land-based inputs into the sea may influence the trophic structure and functioning of coastal and continental shelf ecosystems, despite the numerous opportunities and services the latter offer to humans and wildlife. In addition, hydrological structures and physical dynamics potentially influence the sources of organic matter (e.g., terrestrial versus marine, or fresh material versus detrital material) entering marine food webs. Understanding the significance of the processes that influence marine food webs and ecosystems (e.g., terrestrial inputs, physical dynamics) is crucially important because trophic dynamics are a vital part of ecosystem integrity. This can be achieved by identifying organic matter sources that enter food...
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Ano: 2015 URL: http://archimer.ifremer.fr/doc/00275/38612/37468.pdf
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Some expected impacts of the Common Fishery Policy on marine food webs ArchiMer
Kopp, Dorothee; Robert, Marianne; Chouvelon, Tiphaine; Mehault, Sonia.
For the ecosystem approach to fisheries management, understanding population dynamics and ecosystem resilience in response to the landing obligation acted by the reform of the European Common Fishery Policy (CFP) is currently an important avenue of research. This study attempts to assess the impact of the new CFP on marine food webs. Total carbon and nitrogen loss induced by a discard ban were estimated for the ecosystem of the Bay of Biscay based on French At-Sea Observer data and carbon and nitrogen content of discarded fish. Changes in predator diets were estimated by comparing two Bayesian mixing models, one mimicking the current situation and the second the application of the new CFP (removing the discarded species that would be landed from the...
Tipo: Text Palavras-chave: Fishery discards; Discard ban; Bay of Biscay; Organic input; Resource use.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00308/41895/41229.pdf
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Stable isotope ratios in bentho-demersal biota along a depth gradient in the bay of biscay: A multitrophic study ArchiMer
Schaal, Gauthier; Nerot, Caroline; Grall, Jacques; Chouvelon, Tiphaine; Lorrain, Anne; Mortillaro, Jean-michel; Savoye, Nicolas; Brind'Amour, Anik; Paulet, Yves-marie; Le Bris, Herve.
Although stable isotope ratios are increasingly used to investigate the trophic ecology of marine organisms, their spatial variations are still poorly understood in the coastal environment. In this study, we measured the stable isotope composition (δ13C, δ15N) of suspended particulate organic matter (SPOM) (primary producer), a suspension feeder, the great scallop Pecten maximus (primary consumer), megabenthic decapods and benthic fishes (secondary consumers) along a depth gradient (from 5m to 155m depth) across the continental shelf of the Bay of Biscay. Although the three trophic levels exhibited similar δ13C patterns along the gradient, the δ15N patterns varied between SPOM, scallops and carnivores. The δ15N difference between SPOM and scallops...
Tipo: Text Palavras-chave: Trophic; Coastal; Bivalves; Food web; Bay of Biscay.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00288/39921/38481.pdf
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Spatial and temporal distribution of mercury and methylmercury in bivalves from the French coastline ArchiMer
Briant, Nicolas; Chouvelon, Tiphaine; Martinez, Laura; Brach-papa, Christophe; Chiffoleau, Jean-francois; Savoye, N.; Sonke, J.; Knoery, Joel.
Marine mercury (Hg) concentrations have been monitored in the French coastline for the last half a century using bivalves. The analyses presented in this study concerned 192 samples of bivalves (mussels: Mytilus edulis and Mytilus galloprovincialis and oysters: Crassostrea gigas and Isognomon alatus) from 77 sampling stations along the French coast and in the French Antilles sea. The goals of this study were to assess MeHg levels in various common bivalves from French coastline, and to identify possible geographic, taxonomic or temporal variations of concentrations. We show that the evolution of methylmercury (MeHg) concentrations covary with total mercury (HgT) concentrations. Moreover, in most of the study sites, HgT concentrations have not decreased...
Tipo: Text Palavras-chave: Mercury; Methylmercury; France; Bivalves; Speciation.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00353/46465/46398.pdf
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Tracking, feather moult and stable isotopes reveal foraging behaviour of a critically endangered seabird during the non-breeding season ArchiMer
Meier, Rhiannon E.; Votier, Stephen C.; Wynn, Russell B.; Guilford, Tim; Mcminn Grive, Miguel; Rodriguez, Ana; Newton, Jason; Maurice, Louise; Chouvelon, Tiphaine; Dessier, Aurelie; Trueman, Clive N..
Aim The movement patterns of marine top predators are likely to reflect responses to prey distributions, which themselves can be influenced by factors such as climate and fisheries. The critically endangered Balearic shearwater Puffinus mauretanicus has shown a recent northwards shift in non-breeding distribution, tentatively linked to changing forage fish distribution and/or fisheries activity. Here, we provide the first information on the foraging ecology of this species during the non-breeding period. Location Breeding grounds in Mallorca, Spain, and non-breeding areas in the north-east Atlantic and western Mediterranean. Methods Birdborne geolocation was used to identify non-breeding grounds. Information on feather moult (from digital images) and...
Tipo: Text Palavras-chave: Balearic shearwater; Diet; Discards; Feeding ecology; Fisheries; Migration; Trophic.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00358/46912/46817.pdf
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Chemical contaminants (trace metals, persistent organic pollutants) in albacore tuna from western Indian and south-eastern Atlantic Oceans: Trophic influence and potential as tracers of populations ArchiMer
Chouvelon, Tiphaine; Brach-papa, Christophe; Auger, Dominique; Bodin, Nathalie; Bruzac, Sandrine; Crochet, Sylvette; Degroote, Maxime; Hollanda, Stephanie J.; Hubert, Clarisse; Knoery, Joel; Munschy, Catherine; Puech, Alexis; Rozuel, Emmanuelle; Thomas, Bastien; West, Wendy; Bourjea, Jerome; Nikolic, Natacha.
Albacore tuna (Thunnus alalunga) is a highly commercial fish species harvested in the world's Oceans. Identifying the potential links between populations is one of the key tools that can improve the current management across fisheries areas. In addition to characterising populations' contamination state, chemical compounds can help refine foraging areas, individual flows and populations' structure, especially when combined with other intrinsic biogeochemical (trophic) markers such as carbon and nitrogen stable isotopes. This study investigated the bioaccumulation of seven selected trace metals – chromium, nickel, copper (Cu), zinc (Zn), cadmium (Cd), mercury (Hg) and lead – in the muscle of 443 albacore tunas, collected over two seasons and/or years in the...
Tipo: Text Palavras-chave: Top predator; Bioaccumulation; Inorganic elements; Organic contaminants; Stable isotopes; Intrinsic markers.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00384/49532/51152.pdf
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Biomonitoring along the french coastline: could mercury isotopic composition indicate a temporal change in hg reaching the coastal zone? ArchiMer
Briant, Nicolas; Chouvelon, Tiphaine; Brach-papa, Christophe; Chiffoleau, Jean-francois; Savoye, Nicolas; Sonke, Jeroen; Knoery, Joel.
Mercury (Hg) is a natural element toxic to all living organisms. Its ocean biogeochemical cycle is dominated by atmospheric deposition, which human activities contribute to disrupt signi cantly, and to a lesser extent by riverine discharge. This element is bioamplifed and bioaccumulated in marine food webs. since mercury concentrations in some coastal animal species of high trophic level approach sanitary thresholds, understanding biogeochemical processes and mechanisms leading to these elevated Hg concentrations becomes important. The stable isotopic geochemistry of metal is indeed a very promising way to trace processes and transfers from one mercury biogeochemical reservoir to another (Sonke and Blum, 2013). The stable isotopes of Hg undergo...
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Ano: 2017 URL: http://archimer.ifremer.fr/doc/00394/50561/51253.pdf
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Trophic ecology of commercial-size meagre, Argyrosomus regius, in the Bay of Biscay (NE Atlantic) ArchiMer
Hubans, Barbara; Chouvelon, Tiphaine; Begout, Marie-laure; Biais, Gerard; Bustamante, Paco; Ducci, Lillian; Mornet, Francoise; Boiron, Anne; Coupeau, Yann; Spitz, Jerome.
In the north-eastern Atlantic, meagre (Argyrosomus regius) is one of the largest fish living on the shelf and this species has important commercial interest. Over the past two decades, large variations in meagre abundance have been observed with pluri-annual cycles but the factors involved in such variations are still unclear. Trophic interactions between meagre and other species (both prey and competitors) might be one explanatory variable of the observed variations in meagre recruitment and abundance. In the present study, we described the diet of commercial-size meagre in the Bay of Biscay from stomach content and stable isotope analyses, and explored its dietary ontogeny. We found that commercial-size meagres were mostly piscivorous with a diet...
Tipo: Text Palavras-chave: Diet; SIAR; Ontogeny; Marine top predators; Sciaenidae.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00384/49531/50028.pdf
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Insights from the spatial and temporal distributions of mercury species and isotopes in bivalves from the french coastline ArchiMer
Briant, Nicolas; Knoery, Joel; Chouvelon, Tiphaine; Brach-papa, Christophe; Chiffoleau, Jean-francois; Savoye, Nicolas; Sonke, Jeroen.
Mercury cycles between atmosphere, terrestrial and aquatic reservoirs through a complex combination of transport and transformation processes, until it is buried in oceanic or terrestrial sediments. Monomethylmercury is the main species bioaccumulated and bioampli ed in the food chain. Knowledge of the origin (continental, oceanicorlocalsediment)ofthisspeciesforthelittoralenvironnment can still be improved. This study focuses on ~200 samples of bivalves (mussels: Mytilus edulis and Mytilus galloprovincialis and oysters: Crassostrea gigas and Isognomon alatus) from ~80 stations along the French coast. The goals of this study were i) to describe Hg levels, speciation and isotopic composition in various bioindicator bivalves from the French coastline, and ii)...
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Ano: 2017 URL: http://archimer.ifremer.fr/doc/00394/50562/51254.pdf
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Oligotrophy as a major driver of mercury bioaccumulation in medium-to high-trophic level consumers: A marine ecosystem-comparative study ArchiMer
Chouvelon, Tiphaine; Cresson, Pierre; Bouchoucha, Marc; Brach-papa, Christophe; Bustamante, Paco; Crochet, Sylvette; Marco-miralles, Francoise; Thomas, Bastien; Knoery, Joel.
Mercury (Hg) is a global contaminant of environmental concern. Numerous factors influencing its bioaccumulation in marine organisms have already been described at both individual and species levels (e.g., size or age, habitat, trophic level). However, few studies have compared the trophic characteristics of ecosystems to explain underlying mechanisms of differences in Hg bioaccumulation and biomagnification among food webs and systems. The present study aimed at investigating the potential primary role of the trophic status of systems on Hg bioaccumulation and biomagnification in temperate marine food webs, as shown by their medium-to high-trophic level consumers. It used data from samples collected at the shelf-edge (i.e. offshore organisms) in two...
Tipo: Text Palavras-chave: Biomagnifying metal; Bioaccumulation; Trophic status; Ecosystem productivity; Temperate systems.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00410/52111/52936.pdf
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Trace metal concentrations in the muscle of seven marine species: Comparison between the Gulf of Lions (North-West Mediterranean Sea) and the Bay of Biscay (North-East Atlantic Ocean) ArchiMer
Mille, Tiphaine; Cresson, Pierre; Chouvelon, Tiphaine; Bustamante, Paco; Brach-papa, Christophe; Bruzac, Sandrine; Rozuel, Emmanuelle; Bouchoucha, Marc.
Concentrations of 6 trace metals (Ag, Cd, Cu, Ni, Pb, Zn) in the muscle of 2 sharks (Galeus melastomus and Scyliorhinus canicula), 4 teleosts (Helicolenus dactylopterus, Lepidorhombus boscii, Micromesistius poutassou and Phycis blennoides) and 1 crustacean (Nephrops norvegicus) were compared between the Bay of Biscay (Atlantic Ocean) and the Gulf of Lions (Mediterranean Sea). Although average concentrations and the trace element pollution index were generally higher in the Gulf of Lions, significant differences between the two ecosystems were only found for Zn for Helicolenus dactylopterus, and for Ag and Cu for the crustacean N. norvegicus. Moreover, some relationships between trophic level or size and metal concentrations were found for these two...
Tipo: Text Palavras-chave: Inorganic elements; Contamination; Bioaccumulation; Fish; Shark; Norway lobster.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00448/55958/57497.pdf
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A global perspective on the trophic geography of sharks ArchiMer
Bird, Christopher S.; Verissimo, Ana; Magozzi, Sarah; Abrantes, Katya G.; Aguilar, Alex; Al-reasi, Hassan; Barnett, Adam; Bethea, Dana M.; Biais, Gerard; Borrell, Asuncion; Bouchoucha, Marc; Boyle, Mariah; Brooks, Edward J.; Brunnschweiler, Juerg; Bustamante, Paco; Carlisle, Aaron; Catarino, Diana; Caut, Stephane; Cherel, Yves; Chouvelon, Tiphaine; Churchill, Diana; Ciancio, Javier; Claes, Julien; Colaco, Ana; Courtney, Dean L.; Cresson, Pierre; Daly, Ryan; De Necker, Leigh; Endo, Tetsuya; Figueiredo, Ivone; Frisch, Ashley J.; Hansen, Joan Holst; Heithaus, Michael; Hussey, Nigel E.; Iitembu, Johannes; Juanes, Francis; Kinney, Michael J.; Kiszka, Jeremy J.; Klarian, Sebastian A.; Kopp, Dorothee; Leaf, Robert; Li, Yunkai; Lorrain, Anne; Madigan, Daniel J.; Maljkovic, Aleksandra; Malpica-cruz, Luis; Matich, Philip; Meekan, Mark G.; Menard, Frederic; Menezes, Gui M.; Munroe, Samantha E. M.; Newman, Michael C.; Papastamatiou, Yannis P.; Pethybridge, Heidi; Plumlee, Jeffrey D.; Polo-silva, Carlos; Quaeck-davies, Katie; Raoult, Vincent; Reum, Jonathan; Torres-rojas, Yassir Eden; Shiffman, David S.; Shipley, Oliver N.; Speed, Conrad W.; Staudinger, Michelle D.; Teffer, Amy K.; Tilley, Alexander; Valls, Maria; Vaudo, Jeremy J.; Wai, Tak-cheung; Wells, R. J. David; Wyatt, Alex S. J.; Yool, Andrew; Trueman, Clive N..
Sharks are a diverse group of mobile predators that forage across varied spatial scales and have the potential to influence food web dynamics. The ecological consequences of recent declines in shark biomass may extend across broader geographic ranges if shark taxa display common behavioural traits. By tracking the original site of photosynthetic fixation of carbon atoms that were ultimately assimilated into muscle tissues of 5,394 sharks from 114 species, we identify globally consistent biogeographic traits in trophic interactions between sharks found in different habitats. We show that populations of shelf-dwelling sharks derive a substantial proportion of their carbon from regional pelagic sources, but contain individuals that forage within additional...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00420/53141/54249.pdf
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There's no harm in having too much: A comprehensive toolbox of methods in trophic ecology ArchiMer
Majdi, Nabil; Hette-tronquart, Nicolas; Auclair, Etienne; Bec, Alexandre; Chouvelon, Tiphaine; Cognie, Bruno; Danger, Michael; Decottignies, Priscilla; Dessier, Aurélie; Desvilettes, Christian; Dubois, Stanislas; Dupuy, Christine; Fritsch, Clémentine; Gaucherel, Cédric; Hedde, Mickaël; Jabot, Franck; Lefebvre, Sebastien; Marzloff, Martin; Pey, Benjamin; Peyrard, Nathalie; Powolny, Thibaut; Sabbadin, Régis; Thébault, Elisa; Perga, Marie-elodie.
Trophic ecology is the study of feeding interactions and food acquisition by organisms. It includes the causes and consequences of those behaviours at all levels of biological organisation. As a field of research, it crosses many disciplinary boundaries and provides knowledge that is pertinent to many other areas of ecology. Here we list and categorise the methods available to trophic ecologists whose toolbox has broadened considerably in recent years. They encompass empirical and numerical approaches with focus ranging from molecules to ecosystems. We further examine the relationship of each method to features such as the scale of observation (from microbes to largest organisms) and organisational level (from individuals to ecosystems) as well as the...
Tipo: Text Palavras-chave: Food web; Feeding interactions; Flux of energy; Computer simulations; Trophic models.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00487/59834/65986.pdf
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